FastQ® Direct Real-time PCR

Technical information


Blood Booster for reproducible results directly from blood

  • The FastQ® Direct product line incorporates a Blood Booster Reagent which ensure reliable and highly reproducible results even with difficult blood samples (e.g high EDTA concentration)
  • Blood samples are simply diluted in the buffer and pipetted directly.
  • Saves time, saves money & and reduces risk of cross contamination
  • For performance date click here


Test Principle

  • Ready-to-use reagents and the real-time PCR method provide a simple solution.
  • Amplification from DNA sample with a wide range of concentrations
  • The PCR run takes 90mins
  • The PCR kit uses different fluorophores that are compatible with most common real-time cyclers. We have performed tests on the following devices: 
  • QuantStudio 6 (Thermo Fischer Scientific)
  • CFX 96TM (Bio-Rad)
  • Lightcycler® 480 (Roche)
  • Analysis is performed using the software of the instrument manufacturer.

Product Description

FastQ B*27 direct detects the HLA-B*27 allele group associated with several different autoimmune diseases (Morbus Bechterew or Ankylosing Spondylitis, Reiter’s disease, reactive arthritis) and is, therefore, used as part of the diagnostic process.

  • All CWD B*27 alleles are detected in a single PCR mix
  • Internal amplification control included

Instructions for Use
FastQ B*27 direct

Product Description

FastQ CD direct Coming Soon

  • Detects the relevant DQ alleles associated with coeliac disease in only two PCR mixes using four colours.
  • See table below for alleles detected
  • Internal amplification control

Product Description

FastQ HFE direct Coming Soon

  • Multiplex Real-time PCR for hereditary haemochromatosis
  • Detects the mutations H63D and C282Y for the HFE Gene
  • Internal amplification control

To discuss your purchase requirements in complete confidence, please Contact us using our secure enquiry form, and one of our management team will get back in touch.


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